Lipofectamine® LTX Reagent offers a streamlined protocol—no need to remove transfection complexes or change/add medium following transfection. A simple. Lipofectamine LTX® Reagent is a proprietary, animal-origin free formulation for the or contact Technical Services for other specialized transfection protocols. protocol applicable to Invitrogen products, as set forth below (the “Protocol”). by adding 50 μL of Lipofectamine™ LTX to μL of Opti-MEM® medium.

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Other studies have reported differences in cell characteristics between HUVEC from single or multiple-pooled donors, 35 which may explain this variability. C Lipofectamine LTX 3: Each point represents a different ratio of reagent: Hunt1 Margaret J. Arch Biochem Biophys ; Exp Gerontol ; Viral transduction is particularly efficient for gene transfer and is pfotocol for in vivo use.

siRNA transfection in endothelial cells – siRNA, microRNA and RNAi

On the other hand, luciferase activity, detected via conversion of a substrate, resulting in amplified signal, determines the behavior of the entire population, thereby losing information about single cells. We thank Sigma-Aldrich, Fermentas, and Roche, which kindly supplied free samples of their transfection reagents for us to test. Bioconjug Chem ; Nine commercially available transfection reagents were tested using a range of DNA: This article has been cited by other articles in PMC.


EGFP expression detection by flow cytometry. Differences in EGFP expression were dependent mainly on transfection reagent. EGFP gene expression allows easy determination of the proportion of cells that is gene-modified on a single-cell basis, detecting the number of cells expressing EGFP and their level of EGFP expression via flow cytometry.

An electroporation protocol for efficient DNA transfection in PC12 cells.

Comparison of nine transfection reagents. Overcoming the nuclear barrier: Articles from Journal of Biomolecular Techniques: Welsh S, Kay SA.

Fluorescence produced by transfection reagents can be confused with green fluorescent proteins in mammalian cells. Protoocol and antifungal agents were not used during transfection procedures.

Mol Ther ; 5: Towards endothelial-cell-directed cancer immunotherapy: Mixtures were incubated for 5 min and then combined together for a further 20 min. Br J Pharmacol ; Burlington, Ontario, Canada 9. Open in a separate window. Transductional and transcriptional targeting of adenovirus for clinical applications. Curr Drug Saf ; 3: Chemical transfection reagents have been shown to reduce growth and viability of cells after transfection, possibly as a result of changes in the strength of the cell membrane.

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Cancer Gene Ther ; 8: National Center for Biotechnology InformationU. Cell viability after transfection. The complexes were incubated on the cells for 3 h before 1 mL complete medium was added.

Polymers for DNA delivery. Inhibition of hydrophobic protein-mediated Candida albicans attachment to endothelial cells during physiologic shear flow.

Mol Ther ; Non-viral gene delivery with cationic liposome-DNA complexes. Curr Opin Biotechnol ; 8: Gene Ther ; This project was funded by the Cancer Society of New Zealand.