Article in Ciência Rural 25(2) · January with 4 Reads o herpesvírus suíno (PRV, vírus da doença de Aujeszky) têm sido amplamente utilizadas em vários. doença de Aujeszky em sistema de baculovirus. Régia Maria Feltrin IILaboratório de Sanidade, Embrapa Suínos e Aves, Concórdia, SC, Brasil. IIICentro de. CLONING AND EXPRESSION OF AUJESZKY’S DISEASE VIRUS GLYCOPROTEIN E .. vírus da doença de Aujeszky de surtos em suínos no Estado de Santa.
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Doença de Aujeszky – Wikipédia, a enciclopédia livre
The Sma I restriction endonuclease site ee used for additional specificity confirmation of the amplification products. Especially HVB1 is an important target for specificity assay because is a related herpesvirus which is known to infect swine BHV-1 4. The assay specificity was demonstrated by the absence of amplifications in all heterologous viruses evaluated and in tissue samples derived from seven healthy pigs.
Also, the BLAST search against nucleotide databases of different herpesvirus and random nucleotide sequences revealed this region is very specific for PRV genomes. The analysis directly ddoena clinical samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases. Lanes 1 and 3 are amplification products, Lanes 2 and 4 are amplification products after digestion with Sma I.
Detection of porcine circovirus type 2, porcine parvovirus and porcine pseudorabies virus from pigs with postweaning multisystemic wasting syndrome by multiplex PCR. The analytical sensitivity of the test was estimated to be 1. The development, optimization and evaluation of a polymerase chain reaction PCR assay are presented for the diagnosis of pseudorabies infection.
The polymerase chain reaction PCR is a rapid tool that can be used no only to detect acutely PRV infected pigs but it is suinls recommended test for detect PRV latent infection. A rapid and accurate diagnosis of PRV infection is important for the initiation of appropriate control strategies. Can J Com Med.
Doença de Aujeszky
World Organization for Animal Health. This gene codes for an envelope glycoprotein named gD which plays and important role in binding cellular receptors and is critical for virus replication in different organs aujeeszky Since the rapid detection of infected animals would reduce the potential transmission of the viruses to uninfected herds avoiding the spread of the diseases In addition, positive amplifications were obtained in all the tissue samples, from PRV natural infected pigs, evaluated.
In general, PRV infections must be considered in the differential diagnosis of respiratory, reproductive and nervous disorders. Journal List Braz J Microbiol v. The virus aukeszky replicates in the respiratory tract, spreads along cranial nerves to the brains and via lymph and blood to internal organs, with the reproductive organs being affected.
All the content of the ddoena, except where otherwise noted, is licensed under a Creative Commons License. PRV specific primers were designed using the Oligo 6.
The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases. PCR experiments were performed on serial ten-fold dilutions of a viral suspension of PRV isolate V with a titer of 10 6.
The assay proved to be very sensitive due to as little as 1. The conventional diagnostic procedure is time-consuming and false-negative results may occur in submissions from latently infected animals. Agarose gel electrophoresis was used to detect PCR products. Diagnostic methods for detection of Classical swine fever virus—Status quo and new developments.
This region was highly conserved for all reported genomes as shown se aligning of these sequences. National Center for Biotechnology InformationU. Potential sites of virus latency associated with indigenous pseudorabies viruses in feral swine.
A rapid, sensitive and specific PCR-based diagnostic suonos to detect pseudorabies virus in clinical samples is provided.
Aujjeszky virus principally affects pigs, which are considered to be the natural host for PRV and the reservoir of the virus in nature, but also infects a broad range of wild and non-porcine mammals with the important exception of higher-order primates 8.
Published online Sep 1. Multiplex PCR for the simultaneous detection of pseudorabies virus, porcine cytomegalovirus, and porcine circovirus in pigs. Author information Article notes Copyright and License information Disclaimer.
This assay was based on the amplification of a highly conserved viral gD gene fragment. The isolation and characterization of a strain of infectious bovine rhinotracheitis virus from stillbirth in swine.
Table 1 Primers designed for the specific amplification of the viral gD glycoprotein gene of the PRV genome. The etiological agent of this disease is suid herpesvirus type 1, usually named pseudorabies virus PRVa pantropic alphaherpesvirus which causes fatal infections in baby pigs, respiratory disease and poor growth in fattening pigs and reproductive disorders in adults 28 Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and pigs with emphasis on respiratory tract.
Traditionally, PRV detection is based on direct virus isolation followed by confirmation using immunofluorescence, immunoperoxidase or neutralization tests with specific antiserum 2.
Under typical conditions of intensive swine production, several clinically similar viral diseases can occur which require laboratory differential diagnosis.
Seven virus-negative tissues samples from clinically healthy animals were also included. Negative controls were run with each test. Open in a separate window.
The effect of temperature and oligonucleotide primer length on the specificity and efficiency of amplification by the polymerase chain reaction. The annealing temperature and number of cycles were determined experimentally. Primers designed for the specific amplification of the viral gD glycoprotein gene of the PRV genome. Non-specific reactions were not observed when a related herpesvirus, other porcine DNA genome viruses and uninfected cells were used to assess PCR.
Iowa State University Press; The PCR assay described here provides a rapid, highly sensitive, and cost-effective laboratory diagnosis for pseudorabies infections. Articles from Brazilian Journal of Microbiology are provided here courtesy of Elsevier.