CLSI H 21-A5 PDF

CLSI HA5 Collection, Transport, and Processing of Blood Specimens for Testing Plasma-Based Coagulation Assays and Molecular Hemostasis Assays;. CLSI HA5. Collection, Transport, and Processing of Blood Specimens for Testing Plasma-Based Coagulation Assays; Approved Guideline. HA5 does not provide general guidelines for the performance of coagulation testing. Performance guidelines for specific coagulation assays are addressed.

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Non standardisation of units. Requests to do so should be addressed to the Editor. Whole Blood Analyses S5. Similarly, brown coloured serum due to methaemalbumin, normal results for potassium or the same result from arterial and venous collections should prompt further investigation e.

This decision should be guided by a policy developed in consultation with requesting clinicians.

National Center for Biotechnology InformationU. As the voice of the U. A clear statement describing the magnitude and direction of the interference must be included. Clinically relevant limits can be sourced from: Laboratories must be familiar with best practice collection processes.

Tests of the coagulation system are very sensitive to storage time and temperatureconcentration of anticoagulant, and surface of containers; attention to these parameters is important.

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Consensus Statement for the Management and Reporting of Haemolysed Specimens

Standards Subsctiption may be the perfect solution. Your Alert Profile lists the documents that will be monitored. Influence of hemolysis on routine clinical chemistry cldi.

Proceed to Checkout Continue Shopping. Clin Chem Lab Med. If the document is revised or amended, you will be notified by email. The contents of articles cclsi advertisements in The Clinical Biochemist — Reviews are not to be construed as official statements, evaluations or endorsements by the AACB, its official bodies or its agents.

Find articles by Ken Sikaris. Quantitative Estimation of Haemolysis S2. No literary matter in The Clinical Biochemist — Reviews is to be reproduced, stored in a retrieval system or transmitted in any form by electronic or mechanical means, photocopying or recording, without permission. Please first log in with a verified email before subscribing to alerts. Author information Copyright and License information Disclaimer.

Errors in laboratory medicine. Cut-offs at which assays are affected must be reviewed and updated when analytical platforms are changed. A survey of Australian Laboratories enrolled in the RCPA Quality Assurance Programs confirmed an ongoing variation in the management and reporting of haemolysed samples.

Introduction Haemolysis of blood samples has been documented as the most frequent pre-analytical artefact encountered by laboratories.

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Consensus Statement for the Management and Reporting of Haemolysed Specimens

Laboratories must determine and document at which H-Index level recollection of the sample is appropriate. Appendix Example of a Haemolysis Colour Chart which also shows haemolysis index concentration levels across different analytical platforms Courtesy Goce Dimeski, Pathology Queensland.

The interference can be the result of various mechanisms including: Example of a Haemolysis Colour Chart which also shows haemolysis index concentration levels across different analytical platforms Courtesy Goce Dimeski, Pathology Queensland. Spectral haemoglobin absorbs atand nm – leading to interference with alkaline phosphatase, gamma-glutamyl transferase, total and direct bilirubin.

Enzymatic – release of enzymes which degrade analytes e. Best Practice Collection Technique S1. Laboratories must monitor the frequency of haemolysed blood samples and investigate the source of collection to enable continuous improvement.

This is a useful website featuring a variety of pre-analytical issues with blood collection, sponsored by Becton Dickinson. You may clssi a document from your Alert Profile at any time.