2) In “Chardakov method for water potential measurement” experiment, you find that M solution has not been changed by water loss or absorption by the. Chardakov Technique. Plant Physiology. UNI. Incubating tissue in solution. Pre- incubation. Incubation. Post-incubation. Save for later measurement. Did solution change concentration? Solutes stay the same If water left or entered tissue –Water also entered or left solution –Solution becomes more dilute or.
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Methood 10 mL of water or sucrose 0. Pour off the solutions into a set of empty, correspondingly labeled tubes. Use a cork borer to prepare at least 27 uniform tissue samples from the potato.
Cut them to the same length with a razor blade ca.
A “kink” may be observed charfakov the graph below the x-axis. In both techniques, tissue samples are incubated in a series of solutions of known osmotic water potential. Record the temperature of the solutions Table 1 Using a Pasteur pipet, remove a small amount of water dyed with methylene blue to dye the sucrose solution, dip a dry probe into methylene blue powder and then mix. What is the water potential calculated by this method?
Weigh the cores to the nearest 0. To make this website chadakov, we log user data and share it with processors. About project SlidePlayer Terms of Service. A measure of how much material is packed into a unit volume of the material The fewer. This technique for measuring water potential is similar in theory to the Chardakov method and shares the advantage of being simple to perform and doesn’t require expensive equipment.
In contrast to the Chardakov method which analyzes changes in solution density after incubation, this technique monitors tissue weight changes. Weigh two or preferably three cores, record your data in Table 2 and then place the cores in one of the test solutions. Cytology Osmosis and Water relations.
Incubate the cores for 1. An alternate method to determine his point requires performing a regression analysis of the best fit line of your data.
It is assumed that solute movement between tissue and solution is negligible. Determine the approximate sucrose concentration for which there is no net change in density after tissue incubation i.
Two ways to move water Bulk chardakiv Osmosis Both move water from high energy to low Differences —Source of energy difference. Density changes can be observed by watching whether a drop of the original solution floats or sinks in the test solution after tissue incubation.
Hypertonic Concentration with higher solute concentration and less water concentration Hypotonic lower solute concentration and more water concentration.
Chardakov method Essay – Free Papers and Essays Examples
Which method do you think will be more accurate? Cut them to the same length ca. Change in Weight final – initial g. Boy, Oh Buoyancy Does it Float? Be sure not to include any fragments of the skin. Draw the best fit line for your data.
The water potential measured by this technique should be the same as that obtained the Chardakov method. What is the water potential of potato tissue? Osmosis diffusion of water through a selectively permeable membrane Membrane Chardakv is permeable to water but not to protein Which side has the highest concentration.
Which is more accurate?
Does a gas differ from other fluids? Record your results Table 2 and repeat this procedure for each of the sucrose solutions.