Keywords: Bryophytes, Antibacterial, Antiviral, Cucumber, Pathogenic bacteria, bryophytes for antimicrobial activity. Journal of. Medicinal Plants, 7, ). Antibacterial Activity of Extracts from Some Bryophytes. Vizma Nikolajeva1*, Ligita Liepina1, Zaiga Petrina1, Guntra Krumina1. The antimicrobial activity of aqueous and ethanolic extracts of 11 Bryophyta and Naturally Grown Bryophytes,” Journal of Medicinal Plant Research, Vol. 5, No.

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All extractions were performed with ethanol. Journal of Natural Products, The Bryologist bryo;hytes 2: This suggests that specific antibacterial compound seffective against the selected bacterial species, tend to be isolated more effectively from liverworts and mosses using ethanol. In addition, none of the mosses showed any antibiotic activity when methanolic extractions were used.

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Acknowledgements I would like to gratefully acknowledge Dr. For example, Lunularia cruciata, Plagiochila porelloides and Marchantia polymorphia showed inhibition zones greater than 2.

Plant Products as Antimicrobial Agents. Screening of potential antimicrobial plants requires crude aqueous or alcohol extracts and fine organic extraction methods.

Table 2 Antibiotic activity in disc diffusion assays of bryophytes from South Western British Columbia and reference antibiotics. Marchantia polymorphia Frullania nisquallensis Lepidozia reptans Scapania bolanderi Conocephalum conicum Lunularia cruciata Plagiochila porelloides Porella cordaeana.

All samples were preliminarily washed to remove contaminants. Marchantia polymorphia Frullania nisquallensis Lepidozia reptans Scapania bolanderi Conocephalum conicum Plagiochila porelloides Porella cordaeana Lunularia cruciata.

The outer membrane is responsible for protecting the bacteria from an array of different antibiotics, detergents, and enzymes that would normally damage the inner member or peptidoglycan cell wall However, there have been few studies to screen and confirm the medicinal potential of bryophytes in North Americans for antibiotic activity.

Journal of Ethnopharmacology, Also, these compounds would be subjected to animal and human studies to determine their effectiveness on whole-organism systems, including toxicity and normal microbiota studies.


These values are approximately one third of the inhibition of reference antibiotics. To explore the bryophytic potential in pharmaceutical applications, a quantitative and qualitative screening of S.

Antibiotics were used as positive controls against the bacteria. Methanolic extractions The preliminary results of screening the alcoholic extracts jorunal 14 species of bryophyte tested against three species of bacteria showed that there is a distinct difference between the antibiotic activities of the tested species in relation to the extraction method used.

Physiologically, Gram-negative bacteria have an outer membrane while the Gram-positive bacteria only have peptidoglycan layer Gm —ve Klebsiella pneumoniae. Since different populations of the same species may exhibit different antimicrobial activity, the time and place of collection, and habitat were recorded Table 1. However, few studies have been concerned with journak in-depth analysis of the antimicrobial activity and efficacy of phytochemicals in different species of bryophytes from South Western British Columbia Antibiotic screening of medicinal plants of British Columbian native peoples.

In our study, none of these species displayed any activity. All Rights Reserved Contact Us. International Journal of Antimicrobial Agents, Lastly, Hylocomium jkurnal has shown antibiotic activity against nine Gram-positive bacteria Journal of Botanical Laboratory No.

Antibiotic Activity of Bryophytes. The collections took place from October to November Further investigation would bryophyges required to determine if a polyacetylene, sterol or propolis found in the mosses, and liverworts are responsible to the antibiotic nature of the species. On the other hand, McCutcheon et al states that there are few known natural antibiotic compounds with a high antibacterial activity against Gram-negative bacteria, which parallel our results. Should boys receive the Gardasil Bryophytfs A thorough overview of the fundamentals of Botany.

Bartramia pomiformis, Leucolepsis acanthoneuron, Frullania nisquallensis showed antibiotic activity when ethanolic extractions where used but not when methanolic extracts were used. All extractions were performed with methanol. Mosses and liverworts usually have leaves with a thickness of one cell, except along the costa and the alar cells, which facilitate diffusion Gm -ve Escherichia coli.


Since nearly all of the identified biologically active or antibiotic substances from plants have been identified as aromatic or saturated organic compounds, it was most efficient to obtain the compounds through ethanolic or methanolic extractions.

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A failure to do so could result in an inability to retrieve valuable information, and bryo;hytes dismissing potentially journxl species.

Atricum selwynii and Sphagnum palustre have also shown activity against a variety of Gram-positive and Gram-negative bacterial species 1,2, This includes thallus, sporophyte, aerial gametophyte and underground portions. Reproductive organs were used in identification. Bryophytes in this region are herbaceous plants that grow closely packed together in mats or cushions on rocks, soil, or as epiphytes on the trunks and leaves of forest trees British Columbian bryophytes against Gram-positive and Gram-negative bacteria will be used as evidence for antimicrobial action and efficacy.

Furthermore, this also suggests that one antibiotic compound may be responsible for the antibacterial qualities of a genus or species. The disc diffusion assay technique was used to evaluate antimicrobial activity.

All assays were carried out in triplicates. The homogenous surface liquid of the crude ethanolic extracts was used to impregnate the diffusion discs with antibiotic activity. Lastly, this study aims to determine whether ethanolic and methanolic extracts from similar bryophytes show significant differences in the relative antimicrobial activity.

The occurrence of antibiotic substances appears to be more frequent in hepatics. Eating for Two – The common misconception in pregnancy.

Therefore, homogenous patches were desired with fresh tissue void of dead or dying material.